Postdoctoral Fellows

The Human Technopole Foundation is looking for Post-doctoral fellows to work in the Structural Biology Research Centre in the laboratory of Prof. Alessandro Vannini.

The Vannini Lab combines cryo-electron microscopy (both SPA and cryo-ET), X-ray crystallography, mass spectrometry, single molecule measurements and various biophysical techniques to elucidate the role of multi-subunit macromolecular machineries in genome function and organization. In particular, we are interested in understanding at the molecular level how non-coding gene transcription machineries, auxiliary transcription factors, chromatin remodellers, SMC complexes and transposable elements affect gene expression and genome structure. Typically, we integrate the in vitro findings with biochemical and molecular biology analysis in living cells, using cellular biology approaches, in order to evaluate the impact on human diseases, such as cancer an neurodegenerative diseases.

The HT Centre of Structural Biology has top-notch managed facilities for Cryo-EM (Titan Krios, 1 Glacios, 1 Aquilos2, 1 Spectra, 1 Talos 120), cryo-CLEM, Light imaging, XL- and native mass -pectrometry and support units for Biophysics (mass photometry, SEC–MALLS, ITC, x-ray crystallography etc), Biomass production (E,coli, yeast, insect cells, suspension mammalian cells with fermenters up to 150 litres) and a dedicated managed platform for single molecule experiments on optical tweezers (Lumicks C-trap and M-trap).

We are looking for highly motivated and creative individuals with a strong interest in structural and functional characterization of large multi-subunit complexes, using integrative approaches. Previous expertise in structural biology or, in alternative, RNA biochemistry is essential.

The Post-doctoral fellows will report directly to Prof. Alessandro Vannini, Head of the HT Structural Biology Centre, and will work in close collaboration with members of her team. We encourage international candidates to apply and will support applications to external Postdoctoral fellowship schemes (EMBO, Marie Curie, HPSF, etc.)

PhD in biochemistry, Structural Biology, molecular biology or closely-related disciplines.

• Track record in one or more of the following areas:

– Biochemistry. Experience in recombinant (or endogenous) multi-subunit protein production and purification. Experience in techniques such as isolation of organelles, immunolabeling of cilia and expansion microscopy is a plus.

– Cryo-electron microcsopy. The applicant should be proficient in SPA and/or cryo-ET analisys

– RNA biology. The applicant should be well versed in RNA biochemistry and have a strong background in RNA research .

– Image processing. The applicant should have a strong expertise in cryo-EM SPA analisys.

• Fluency in English – HT is an international research institute.

To apply please provide the following:

• CV with a list of publications.

• A motivation letter

• Names and contacts of 3 referees.

Additional Information:

HT offers a highly collaborative, international culture. The working language at HT is English. HT will foster top quality, interdisciplinary research by promoting a vibrant environment consisting of independent research groups with access to outstanding graduate students, postdoctoral fellows and core facilities.

HT is an inclusive, equal opportunity employer offering attractive conditions and benefits appropriate to a leading, internationally competitive, research organization and seeks to promote a collegial and open atmosphere.

Please note that researchers coming to Italy for the first time, or returning after residing abroad, benefit from very attractive income tax reductions (up to 90%).

General enquires concerning the role should be sent to Prof. Alessandro Vannini ([email protected]>). Applications should not be sent to this address.